T7 IVT Helper

JSON API available here

PCR Primers (click to copy)

T7 Promoter Fwd Primer T7 Terminator Rev Primer
TAATACGACTCACTATAGGG GCTAGTTATTGCTCAGCGG

Enter desired RNA sequence:

Invalid sequence. Please enter only A, C, G, T, or U bases.

T7 IVT ssDNA Template (click to copy)

Output DNA will appear here...

Resulting RNA Sequence (click to copy)

Output DNA will appear here...